Early pregnancy molting membrane accumulation stem cells combined with Dandanhanone IIA to treat rats adhesion

Interpretation of text:

Human early pregnancy molting stem cells: derived from the normal uterine molting tissue, which can secrete a variety of proteins during the development of the placental membrane, and it is powerfulProliferation ability.The study uses collagen enzyme digestion to separate the germinated stem cells between the early pregnancy molting membranes, and the detection of streaming cell meters can express the mesenchymal stem cell surface logo CD90, CD73, and CD105.

Tanzhenone IIA: Tannanone is a fat -soluble Philippiosaurus compound extracted from the Chinese medicine Salvia (Lip -shaped Plants Salvia Miltiorrhiza Bunge root), which is divided into golf tannate Ⅰ, tannsin ⅡA, danda ketone ⅡB, hidden ginseng ketone, and alienThere are more than 10 tannone monomers such as dattosone.Tamanol II is the representative of the fat -soluble components in Danshen. It is concentrated in the skin of the Salva root, which is often used as tumor, cardiovascular disease, inflammation, atherosclerosis, fibrosis and other diseases.

Background: Performance stem cells and dandanone ⅡA between people’s early pregnancy molten membranes have anti -adhesion effects and have a certain effect on the uterine cavity adhesion, but their specific mechanisms are further studied.

Objective: Explore the effects and mechanisms of germination of germinated stem cells combined with Tanzhenone IIA in the early pregnancy of people.

Methods: The collagense Ⅰ digestive method is used to separate the people’s early pregnancy molten stem cells, and 45 female 4-6 weekly SD rats are selected. They are randomly divided into 5 groups. Each group is 9, respectively., Danshanone group, stem cell group and combined treatment group.Fake surgery in the control group; the model group was not intervened after the mold was made; the stem cell group injected 0.2 ml by the palace after the mold was created., Every 3 d injection, 3 times in total; Tannanone group 7 d spilt -in 11 mg/(kg · d) Tanfantone IIA solution for 1 week after the mold is made, and the stomach is continued for 1 week; the combined treatment group is the above two treatments.Combination of methods.The control group and the model group were taken after 5, 10, and 15 days after surgery, and each treatment group took the material after the last treatment 5, 10, and 15 d.Degree; ELISA detects the level of serum estrogen E2 and vascular endothelial growth factor; Q-PCR method detect MIR-29A MRNA expression in uterine tissues; Western blot detection of uterine tissue conversion growth factor β1, SMAD3, and matrix metal protease 9 protein expression.

The co -therapy group’s endometrium fibrosis area ratio decreased significantly (P <0.05), while the thickness of the endometrium and the number of glands increased significantly (P <0.05), the stem cell group and the tannosterone group’s mice endometrial fibrosis ratio ratio ratio ratioIt also decreased significantly (P <0.05); ② Compared with the model group, the level of serum estrogen E2 E2, the serum estrogen E2 of the Tanzanone group, stem cell group and combined therapy group (P <0.05), the level of vascular endothelial growth factor decreased significantly (P <0.05), MIR-29A MRNA expression significantly increased (P <0.05), the expression of the combined treatment group conversion growth factor β1, SMAD3 protein expression significantly decreased (P <0.05), and the diameter metal protease nine protein expression of the Dansalone group increased significantly (P <0.05);③ The results show that the combined treatment of germination of germinated stem cells between people with early pregnancy molting membranes can effectively repair the endometrium of rats. Its mechanism may reduce the level of conversion growth factor β1 and SMAD3 protein levels by regulating MIR-29A, thereby improving fibrosis.

Sumid language: Human Decidual Mesenchymal Stem Cells, HDMSCS

https://orcid.org/0000-0001-8956-6622 (Xu Weijun)

China’s Organizational Engineering Research Magazine Publishing Content: Stem cells; Bone marrow stem cells; hematopoietic stem cells; fat stem cells; tumor stem cells; embryo stem cells; umbilical cord blood stem cells; stem cell induction; stem cell differentiation; tissue engineering; tissue engineering; tissue engineering; tissue engineering; tissue engineering; tissue engineering;

Keywords: uterine cavity adhesion, early pregnancy molt stem cells, dandanone Ⅱa, miR-29A, conversion growth factor β1, smad3

Quoting this article: Xu Weijun, Hu Xiangdan, Yu Dongqing. Early pregnancy Mulfids combined with Dandshutlthone IIA for the treatment of rats to treat the uterine cavity [J]. China Organization Engineering Research, 2022, 26 (25): 3986-3992.

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Early pregnancy molting membrane accumulation stem cells combined with Dandanhanone IIA to treat rats adhesion

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